GEO 数据集

主题由标题与摘要自动归类；样本量为数据集标注之和，SuperSeries 可能与其 SubSeries 重复计算。

• An Engineered Tn5 Variant Enhances CUT&Tag Fidelity for Transcription Factors
  GSE351354Homo sapiens; Mus musculus357 个样本2026-10-26

  The CUT&Tag method performs exceptionally well for profiling histone modifications, but mapping sequence-specific transcription factors (TFs) has been more challenging. This is because the Tn5 transposase used in CUT&Tag has an intrinsic affinity for DNA, requiring high-salt washes to suppress non-specific signal at accessible sites, but these stringent ionic conditions can also disrupt TF–chromatin interactions. Here, we develop an improved TF-CUT&Tag strategy to capture these salt-sensitive TF binding sites. We engineered the Tn5 DNA-binding channel by neutralizing positively charged residues and identified the K160A mutation as improving CUT&Tag specificity for GATA2, as measured by enrichment of TF DNA-binding motifs under physiological NaCl and KCl-based DynaTag conditions. Applying this improved assay to the Yamanaka pluripotency TFs in mouse embryonic stem cells increased recovery across their genomic binding landscapes while preserving factor-specific motif architectures. During skeletal muscle differentiation, TF-CUT&Tag revealed strongly motif-enriched MyoG binding and a shift in Pax7 occupancy from CpG-rich promoters in progenitors toward Pax7 motif-enriched sites during differentiation. Together, these results show that TF-CUT&Tag captures distinct modes of TF–chromatin interaction, including motif-directed binding sites and more salt-sensitive sites that may depend on lower-affinity DNA recognition or protein–protein interactions and are difficult to predict from DNA sequence alone.